【摘要】 目的 :选用人脐静脉内皮EA .hy 92 6细胞株作为研究对象 ,观察二甲基亚砜溶解的香烟尘粒 (DSP)对人脐静脉内皮EA .hy 92 6细胞生长的影响。方法 :以 (1、2、4、8)mL/L剂量DSP作量效实验 ,小剂量DSP(2mL/L)作时效实验 ,采用MTT比色法和 96孔板细胞蛋白测定方法来评价DSP对该细胞株增殖和活性的影响。透射电镜(TEM)观察不同处理因素作用后细胞超微结构变化。结果 :DSP能抑制人脐静脉内皮EA .hy 92 6细胞增殖 (P <0 0 5 ) ,且对该细胞株具有明显毒性 ,它能减少细胞蛋白合成 (P <0 0 5 )、增加细胞死亡 (主要为坏死 ) ,作用呈剂量和时间依赖。结论 :DSP能损伤血管内皮细胞 (VEC)。更多还原

【Abstract】 AIM: To investigate the injuring effect of DMSO-soluble particles from cigarette smoke(DSP) on human umbilical vein endothelial cells. METHODS: Human umbilical vein endothelial cell line EA. hy 926 was used as target cells in the study. The growth and viability of the cells treated with various dosages (1, 2, 4 or 4 mL/L) of DSP and low dose (2 mL/L) of DSP at different time points were evaluated by MTT colorimetric assay and celllular protein assay in 96-well plates. Transmission electron microscopy study was carried out to observe the ultrastructure of human umbilical vein endothelial cells under DSP treatment.RESULTS: DSP inhibited the proliferation of human umbilical vein endothelial cell line EA. hy 926. Under DSP treatment, the reducing cellular protein and increasing cell death(mainly necrosis) were observed in time-dependent and dosage-dependent manners.CONCLUSIONS: These results indicated that the toxic effect of DSP caused functional disturbance and structural damage of human endothelial cells.更多还原