【摘要】 目的 :建立HHV - 6BU83转基因小鼠系 ,为进一步探讨HHV 6BU83基因与多发性硬化的关系提供动物实验模型。方法 :转基因动物技术。结果 :通过构建含有导引神经系统星型细胞特异表达启动子及小鼠鱼精蛋白 1的HHV - 6BU83DNA片段 ,将其显微注入小鼠受精卵后 ,建立了 3个HHV - 6BU83转基因小鼠系。结论 :建立人类疱疹病毒 6BU83转基因小鼠系 ,对于研究观察HHV - 6B感染与神经系统疾病的关系具有重要意义。更多还原

【Abstract】 AIM: Human herpesvirus 6B(HHV-6B) is the etiologic agent of exanthem subitum and is associated with febrile illnesses in children, neurological manifestations during primary infection, and clinical complications in organ transplant patients. The ORF U83 of HHV-6B has been identidied encoding a functional chemokine and U83 protein was detected out in brain tissues of some patients with multiple cirrhosis. The aim of this study was to establish HHV-6B U83 transgenic mouse lines, which would provide a pathological animal model for the research of the relationship between HHV-6B U83 gene and multiple cirrhosis. METHODS: Transgenic animal technique was applied in the present study. RESULTS: We successfully established 3 lines of transgenic mice with HHV-6B U83 gene integrated to their chromosomes, through constructing the DNA fragment which consisted of GFAP promotor, HHV-6B U83 and mouse protamine 1 sequence and injecting to the mouse eggs. CONCLUSION: This establishment of HHV-6B U83 transgenic mouse lines would benefit the observation and analysis of association of HHV-6B infection with neurological system diseases.更多还原