【摘要】 目的 :观察脊髓损伤 (SCI)后内皮素 - 1(ET - 1)mRNA表达的解剖和空间以及含量变化规律。方法 :改良Allen’s法 ( 5 0g -cm)制备急性大鼠脊髓挫伤模型。原位杂交检测SCI前后脊髓组织中ET - 1mRNA。图像分析半定量测定伤区邻近段ET - 1mRNA含量。结果 :SCI后损伤邻近段脊髓组织中ET - 1mRNA阳性表达的神经元、胶质细胞与血管内皮细胞数量多于及信号强于对照组 ,以神经元表达改变为主。除 4 8h组 ,伤后各时点脊髓灰质ET - 1mRNA阳性染色的阳性单位与对照组比较差异显著 (P <0 .0 5 )。伤区脊髓中ET - 1mRNA阳性染色神经元随时间逐渐减少 ,而胶质细胞阳性表达增加。结论 :SCI后脊髓组织中ET - 1mRNA表达上调 ,提示ET - 1参与SCI后病理生理改变。神经元是SCI后脊髓组织中ET - 1升高的主要来源更多还原

【Abstract】 AIM: To determine the topographic and spactial changes of endothelin-1 (ET-1) mRNA in cords after spinal cord injury(SCI). METHODS: A SCI model of the rat was made by modified Allen’s weight drop method(50g-cm). ET-1 mRNA in the spinal cords before and after injury was examined by in situ hybridization and the content of ET-1 mRNA was determined by semi-quantitative image pattern analysis. RESULIS: Compared with control, the ET-1 mRNA positive neurons, glial cells and endothelial cells increased and the positive signal enhanced in the adjacent cord of the contused region. There was also a significant increase in positive unit of ET-1 mRNA staining in injured spinal cord except 48 h-group. The quantity of neuron expressing ET-1 mRNA decreased gradually in contused region after SCI, while the quantity of glial cell expressing ET-1 mRNA increased. CONCLUSION: Expression of ET-1 mRNA upregulates in spinal cord after SCI. It suggests a pathophysiological role for ET-1 in SCI. Neuron is the main contributor to the increase for ET-1 in injured spinal cord.更多还原