【摘要】 依据马铃薯S病毒 (PotatovirusS ,PVS)外壳蛋白 (CP)基因序列 (885bp)设计合成了两对引物 ,通过RT PCR扩增得到长 0 .8kb的目的片段 ,将目的片段转入大肠杆菌 ,酶切鉴定证明得到了含有目的片段的重组子 ,测定序列结果与其他PVS分离物CP基因的序列比较 ,发现其核苷酸同源性达 95 %左右 ;构建了含PVSCP基因的融合蛋白原核表达载体 ,并在大肠杆菌中得到表达 ,SDS PAGE测定融合蛋白的分子量为 5 8kD。更多还原

【Abstract】 Based on the PVS coat protein gene sequence (885bp) a pair of specific primers were designed and the coat protein (CP) gene of potato virus S(PVS) was amplitied by RT PCR.The gene was cloned into pGEX 2T vector and then transformed into \%E.coli\% DH5α.The gene has high homology (95%) comparing with other isolate genes.The expression vector p2TSCP was constructed.The coat protein gene was expressed in \%E.coli\% DH5α and a 58kD protein was analysed by SDS PAGE.更多还原