【摘要】 本试验以转化CMV CP和TMV CP基因的转基因线辣椒纯合系植株作为研究试材 ,比较了单独或混合接种CMV和TMV后 ,转化线辣椒的抗病性表达特点 ,并测定了两种病毒在植株体内的病毒含量。结果表明 :转化线辣椒不仅能抵抗CMV和TMV的单独侵染 ,而且还能抵抗CMV和TMV的复合侵染。转化线辣椒表现为系统症状延迟出现 7 15d ,显症株率和病害严重度级别大幅度降低 ,CMV和TMV在接种叶、新生叶中的病毒含量明显减低。转基因线辣椒原生质体作为研究试材接种CMV ,测定病毒含量结果表明 :CMV病毒的增殖在转基因线辣椒原生质体内受到明显抑制。在CMV接种浓度为 4 0 μg/mL ,感染原生质体 4 8h后 ,CP(- )植株原生质体内CMV是CP(+)的 4 .2倍。这一结果揭示了转基因线辣椒具有抑制病毒增殖的抗病性。更多还原

【Abstract】 Transgenic chili pepper plant expressing CMV and TMV coat protein gene were infected with CMV and TMV to comparing the characteristic of CP MR in transgenic chili pepper and virus content in inoculated plants.The results showed that the transgenic chili pepper exhibited high level of coat protein mediated resistance to both CMV and TMV.The development of systemic symptom was 7～15 days delayed,percentages of plants showing symptoms and severity of disease symptom in inoculated plants were strongly reduced,and virus accumulat and spread were obviously repressed in inoculated and newly developed leaves in CP(+) plants challenged with CMV and TMV.The protoplasts from transgenic chili pepper expressing CMV CP and TMV CP were infected with CMV virion.The results showed that protoplasts from transgenic chili pepper can also inhibit the virus proliferation.The protoplasts were infected with 40μg/mL virus concentration,48 hours later,the amount of virus in control plant protoplasts was 4.2 times than that in CP(+) plant protoplasts.This results demonstrated that the resistance characteristic of transgenic chili pepper was to reduce virus proliferation in plants.更多还原