【摘要】 为了了解云南辛德毕斯病毒在BHK2 1细胞上的生长特性 ,并观察该病毒在BHK2 1细胞、3d龄和 2周龄小白鼠中是否有凋亡反应出现。在接种后不同时间取样 ,检测病毒的滴度 ,电镜检测凋亡细胞 ,检测细胞的DNA阶梯。一步生长曲线结果表明病毒在出现CPE之前就有大量繁殖。秋水仙素对该病毒的成熟释放没有抑制作用。该病毒对BHK2 1、小白鼠均可产生细胞凋亡更多还原

【Abstract】 The one step growth curve of YN87448 strain virus in BHK21 cell showed that a lot of progeny viruses could be observed at 16 hr. after infection. The titer of virus reached the highest about 10\+\{-7.0\}TCD50 at 48 hour post infection. The titer of virus is not reduced by Col. The apoptosis was observed by the electron microscope and the DNA ladder was detected by agarose gel eletrophoresis. YN87448 strain virus can cause apotosis in BHK21 cell and mice.更多还原