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香榧主要栽培品种的RAPD分析

Molecular Identification of Main Cultivars of Torreya grandis by RAPD Markers

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【作者】 谭晓风胡芳名张党权周煦惠杨伟

【Author】 Tan Xiaofeng,Hu Fangming,Zhang Dangquan,Zhou Xuhui,and Yang Wei (The Key laboratory of Non wood Forest Tree Breeding and Cultivation of Forestry Ministry,Zhuzhou 412006)

【机构】 中南林学院经济林育种与栽培国家林业局重点实验室中南林学院经济林育种与栽培国家林业局重点实验室 株洲412006株洲412

【摘要】 以香榧 8个主要栽培品种无性系的种子胚乳DNA为研究材料 ,利用 2 3种多态型明显的引物进行了RAPD分析。结果表明 :8个品种之间在DNA水平上表现出较大的异质性 ;根据RPAD特异分离谱带确定了各品种的标记基因型 ,以此可实现对香榧无性系品种DNA水平上的鉴别 ;以标记基因型为依据对香榧 8个品种进行系统聚类分析 ,结果与传统形态学分类中的两大类基本吻合

【Abstract】 The endosperm DNA samples of the seeds of a same individual plant of eight main cultivars (clones) of Torreya grandis are analyzed with RAPD technology by using 23 primers that have distinct polymorphism?The result indicated that there was a relatively large difference between the eight varieties at the level of DNA molecule; the marker genotype of varieties was established according to particularly isolated bands in the experiments; the clone varieties of Torreya grandis can be identified at the level of DNA molecule according to the marker genotype; the eight varieties of Torreya grandis were analyzed systematically with a assembling analysis technology, and the analytic result tallied with the two classes of traditional morphological classification on the whole.

【基金】 国家林业局重点课题资助项目 (2 0 0 0 -3 1)
  • 【文献出处】 园艺学报 ,Acta Horticulturae Sinica , 编辑部邮箱 ,2002年01期
  • 【分类号】S791
  • 【被引频次】63
  • 【下载频次】212
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