【摘要】 以双丰等 17个辣椒品种为试材 ,探讨了不同基因型、激素组合、有机成分及AgNO3 等因素对子叶再生的影响。观察到DJ添加物 (辣椒幼苗茎叶提取汁液∶卡那霉素 =5 0 0∶1)对不定芽的分化及伸长有明显的促进作用 ,比对照分别提高 10 .0 %～ 11.1%和 90 .5 %～ 133.3%。筛选出高效芽分化培养基为MB+IAA 1.0mg/L +6 BA 5 .0mg/L +DJ 5 0 0 0 .0mg/L +AgNO3 10 .0mg/L ,17个品种平均芽分化率达 92 .3% ;高效芽伸长培养基为MB +IAA 1.0mg/L +6 BA 5 .0mg/L +DJ 5 0 0 0 .0mg/L +AgNO3 10 .0mg/L +GA3 2 .0mg/L ,17个品种平均芽伸长率达 5 7.8% ;高效生根诱导培养基为MS +IAA 0 .2mg/L +NAA 0 .1mg/L ,生根率达 90 % ,建立了辣椒子叶高效植株再生体系更多还原

【Abstract】 The report describes the development of a high efficient in vitro regeneration procedure from cotyledon explants of 17 sweet/chilli pepper cultivars(Shuangfeng et al).The effect of genotype, hormone, organic nutrient component and AgNO 3 et al on the tissue culture from pepper cotyledon explants was investigated. Adding DJ(juice of peppr stem and leaf+kanamycin=500∶1) organic nutrient component can improved the shoot buds differentiation and elongation rate. The best media for in vitro regeneration from cotyledon explants of pepper were discovered: the shoot buds induction medium:MB+IAA 1.0mg/L+6 BA 5.0mg/L+DJ 5.0g/L+AgNO 3 10.0 mg/L; the shoot buds elongation medium:MB+IAA 1.0mg/L+6 BA 5.0mg/L +DJ 5.0g/L+AgNO 3 10.0mg/L+GA 3 2.0mg/L; the rooting medium:MS+IAA 1.0mg/L+NAA 0.1mg/L.更多还原