【摘要】 磷钼杂多酸与蛋白质反应形成粒径为 80 nm的复合纳米微粒 ,在 470 nm处产生一个共振散射峰。该纳米微粒和界面的形成是导致散射光信号增强的根本原因。在选定条件下 ,建立了一个线性范围为 6.0×10 -8～ 5 .0× 10 -5g/ m L 及检出限为 2 .0× 10 -8～ 1.0× 10 -7g/ m L 的测定人血清白蛋白和酪蛋白的共振散射光谱新方法。该法具有灵敏度高、操作简便、准确度好、测定范围宽等特点 ,已用于合成样品和人血清样品分析 ,结果令人满意。更多还原

【Abstract】 A maximum resonance scattering(RS) peak at 470 nm for the system of protein phosphomolybdic acid(PMA) was observed. The enhanced resonance scattering signal appeared to be the formation of protein PMA composite nanoparticles with diameter about 80 nm. A resonance scattering spectroscopic method has been developed for the quantitative determination of human serum albumin (HSA) and casein. In the condition selected, the value of I 470 nm was found linear to the concentration of protein in the range of 6 0×10 -8 ~5 0×10 -5 g/mL, with detection limit about 2 0×10 -8 ~1 0×10 -7 g/mL. The method has advantages of easy operation, high sensitivity, high accuracy and wide determinination range. Proteins in synthetic samples and human serum samples have been determined by this RS method with satisfactory results.更多还原