【摘要】 目的:建立用高效液相色谱快速检测拉米夫定血药浓度的方法。方法:用10％高氨酸和20％三氯乙酸直接沉淀血清蛋白,三氯甲烷抽提血清杂质成分,离心后取上清液进行色谱分析;色谱柱为Shim-Pack VP-ODS柱,流动相为0.02mol·L^-1磷酸盐缓冲液（pH7.0）-乙腈-甲醇（91:0.1:9）,流速为1.5mL·min^-1,紫外检测波长274nm,喷昔洛韦为内标。结果:拉米夫定的保留时间为7.9min,定量线性范围0.050～4.0mg·L^-1（r=0.9998）,方法回收率大于94.60％（n=5）。日内RSD<4%,日间RSD<7%,n=5。结论:本法简便快速、定量准确,适用于拉米夫定人体临床药代动力学和生物利用度的研究。更多还原

【Abstract】 Objective: A specific HPLC method was developed for the measurement of lamivudine in human serum. Methods: The serum protein was precipitated by the addition 10% of perchloric acid and 20% trichloroacetic acid. Chloroform was used to extract the interfering materials in the serum. The resulting supernatant liquid was directly injected after centrifuge. The chromatography condition were: Shim - Pack VP - ODS column, 0.02 mol·L-1 phosphate buffer (pH7.0) - acetonitrile - methanol (91:0.1:9) as mobile phase at 1.5 mL·min-1. Detection wavelength was 274 nm. Penciclovir was the internal standard. Results: The retention time of lamivudine and internal standard were 7.9 min and 6.2 min respectively. A good linearity was shown in the concentration range of 0.050 - 4.0 mol·L-1 ( r = 0.999 8) . The recovery was more than 94.60% . The intra - day RSD was less than 4% and the inter - day RSD was less than 7% ( n = 5). Conclusion: This method is simple, rapid and accurate. It was applied to study of pharmacokinetic and bioavailability of lamivudine.更多还原