【摘要】 目的 :研究 1 甲基 4 苯基 吡啶离子 (MPP+)和 6 羟基多巴胺 (6 OHDA)对C6胶质瘤细胞摄取谷氨酸 (Glu)的影响。方法 :应用放射免疫法测定C6胶质瘤细胞对培养液中 [3H] D ,L 谷氨酸的摄取 ;应用四氮唑 (MTT)比色法检测胶质瘤细胞的活性。结果 :6 OHDA和MPP+均不同程度地抑制C6胶质瘤细胞摄取Glu ;6 OHDA显著抑制C6胶质瘤细胞活性 ,而MPP+却不影响细胞活性 ;蛋白激酶C(PKC)激动剂TPA显著增强C6细胞对Glu的摄取 ,并拮抗MPP+对C6细胞摄取Glu的抑制。结论 :6 OHDA抑制C6胶质瘤细胞对Glu的摄取 ,可能与其抑制细胞活性有关 ,而MPP+的抑制作用可能与直接影响转运体的摄取功能有关 ,并可能由PKC途径介导更多还原

【Abstract】 AIM: To study the effects of 6 OHDA and MPP + on the glutamate uptake into cultured C6 glioma cells. METHODS: The glutamate uptake into C6 glioma cells was measured by using radioimmunoassay method; and the viability of C6 glioma cells was investigated by MTT method. RESULTS: Both 6 OHDA and MPP + inhibited glutamate uptake into C6 glioma cells in a concentration dependent manner. Treatment with 6 OHDA produced inhibitory effect on the viability of C6 glioma cells. However, exposure to MPP + showed no such effect. Incubation with TPA, a PKC activator, caused increase in glutamate uptake and completely reversed MPP + induced uptake inhibition. CONCLUSION: Inhibition of glutamate uptake by MPP + was completely reversed by TPA, indicating that the effect of MPP + was mediated by PKC cell signal transduction, while the inhibitory effect of 6 OHDA was due to the impairment viability of C6 glioma cells.更多还原