【摘要】 目的 探讨凋亡相关蛋白TFARl9在老年性白内障晶状体上皮细胞中的表达。 方法 以TFAR19蛋白的单克隆抗体为工具,利用过氧化酶标定的链霉卵白素染色试剂盒对老年性白内障晶状体上皮细胞行免疫组化染色,观察TFAR19蛋白在晶状体上皮细胞中的表达及定位。结果 发现正常人品状体上皮细胞中有TFAR19蛋白低水平的表达,老年性白内障晶状体上皮细胞中有3种高表达的细胞:一种为少部分细胞呈胞浆高表达,胞核不表达;另一种为偶见胞核、胞浆均高表达;第3种为凋亡晚期的细胞仅在凋亡小体上有表达。 结论 老年性白内障晶状体上皮细胞中有凋亡相关蛋白TFAR19的特征性表达,该结果对进一步揭示细胞凋亡是否参与自内障的发病有着重要意义。更多还原

【Abstract】 Objective To study the expression of apoptosis-related protein TFAR19 in human lens epithelial cells from senile cataract patients. Methods Utilizing monoclonal antibody against TFAR19,the expression level and localization of TFAR19 were examined by biological microscope. Results There was low expression of TFAR19 protein in normal cells. The expressing cells were divided into three kinds:1 there was strong expression in cytoplasm, but no expression in nucleus;2 there was expression in both cytoplasm and nucleus and the last one was that TFAR19 only can be found in apoptosic body. Conclusions Apoptosis-related protein TFAR19 is characteristically expressed in human lens epithelial cells from senile cataract patients. This result is very value for revealing the relation between apoptosis and cataract formation.更多还原