【摘要】 目的探讨视网膜神经节细胞（ＲＧＣｓ）的多种培养方法。方法（１）在涂有鼠尾胶原的器皿上通过调整培养液培养：①乳鼠混合及纯化的ＲＧＣｓ；②单层星型胶质细胞上种植纯化的乳鼠ＲＧＣｓ；③引产胎儿混合ＲＧＣｓ。（２）检验鼠尾胶原及中脑顶盖提取液（Ｔｅ）对培养的乳鼠ＲＧＣｓ的影响。结果（１）混合培养的乳鼠及引产胎儿ＲＧＣｓ大多能存活２周以上；（２）纯化的乳鼠ＲＧＣｓ大多能存活４８ ｈ；（３）单层星型胶质细胞上种植的纯化乳鼠ＲＧＣｓ能存活４周以上；（４）鼠尾胶原及Ｔｅ对培养的乳鼠ＲＧＣｓ的贴壁及生长有明显的促进作用。结论通过调整培养液及改善微环境能用多种方法培养ＲＧＣｓ。更多还原

【Abstract】 ObjectiveTo study different culture methods of retina ganglion cells (RGCs).Methods(1)To culture RGCs on utensils that smeared rat’s tail collagen with culture solution:①co-cultivation and pure culture of newborn rat’s RGCs;②culture purified RGCs of newborn rat on subculture monolayer astrocyte;③co-culture of induced labor fetus’s RGCs.(2)To determinate effect of Te and rat’s tail collagen on RGCs.Results(1)Most cocultivation of newborn rat’s RGCs and induced labor fetus’s RGCs could survive for more than 2 weeks;(2)Most of newborn rat’s RGCs could survive for 48 hours;(3)culture purified RGCs of newborn rat on subculture monolayer astrocyte of rat brain could survive for more than 4 weeks;(4)To smear rat’s tail collagen on cell culture utensils and using Te (retinal ganglion neuronotrophic facter,RGNTF) could benefit RGCs to survive.ConclusionRGCs could be cultured on rat’s tail collagen with culture solution of DMEM,N3,bovine serum and Te.更多还原