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水稻无内稃突变体的遗传分析和基因定位
Genetic Analysis and Molecular Tagging of a Gene for Non-palea in Rice
【摘要】 花器官发育异常的突变体是研究植物花发育分子遗传机制的良好实验材料。以水稻无内稃突变体为父本 ,生 4 7、N6 2 5和CDR2 2为母本配制杂交组合进行性状遗传分析 ,根据F2 代表型及 χ2 测验结果表明 ,突变性状是由单隐性基因控制的。选用突变体为父本、生 4 7为母本杂交的F2 群体作定位群体 ,利用SSLP标记和RFLP标记将与突变性状相关的基因定位在第 6染色体短臂上RFLP标记C4 98和RZ4 5 0之间 ,暂定名为npa 1。为进一步的基因克隆及功能研究奠定了基础
【Abstract】 The mutants involved in the development of floral organ are good material for understanding the molecular genetic mechanisms of floral development. A rice mutant, that lacks palea in its florets, was derived from a spontaneous mutation in an indica line, SARⅢ 93 369. Genetic analyses in three F 2 populations from the mutant crossed with three rice varieties, Sheng47, N625 and CDR22, respectively, showed that the mutant trait is controlled by a single recessive gene. In the F 2 population from npa 1/Sheng47 the gene for the non palea trait was mapped between two restriction fragment length polymorphism markers, C498 and RZ450, with distances of 7.5cM and 2.4cM, respectively. The tagged recessive non palea gene is temporarily designated npa 1.
【Key words】 rice ( Oryza sativa ); floral organ; development; genetic analysis; gene localization;
- 【文献出处】 遗传学报 ,Acta Genetica Sinica , 编辑部邮箱 ,2002年03期
- 【分类号】S511.03
- 【被引频次】38
- 【下载频次】215