【摘要】 应用荧光原位杂交 (FISH)技术检测了昆明山海棠 (THH)水抽提物在小鼠生殖细胞形成过程中对 8号染色体不分离的诱发效应。以THH水抽提物 (12 0mg/kg、2 4 0mg/kg、4 80mg/kg)腹腔注射昆明种雄性小白鼠 ,于处理 2 2天后取小鼠附睾精子涂片 ,以生物素标记的 8号染色体着丝粒重复序列探针进行FISH ,结果发现 ,THH在中间剂量组(2 4 0mg/kg)和高剂量组 (480mg/kg)诱发精子 8号染色体非整倍体频率均显著高于溶剂对照 (P <0 .0 0 1) ,低剂量组(12 0mg/kg)的异常精子频率与溶剂对照无显著差异 (P >0 .0 5 )。研究表明 ,THH在雄性小鼠生殖细胞发育过程中具有染色体不分离诱发效应。更多还原

【Abstract】 Fluorescence in situ hybridization (FISH) was employed to detect non disjunction of chromosome 8 during meiosis in young adult male mice after treated by Tripterygium hypoglaucum (Lévl.) Hutch(THH). The Kunming male mice were given THH(120mg/kg,240mg/kg,480mg/kg) by abdominal cavity injection,and sacrificed 22 days sfter the treatment of THH. Epididymis sperm was used to make smear. FISH using bio 16 dUTP labelled DNA probes specific for chromosome 8 was performed on epididymal sperm decondensed for 60min each in 10mmol/L DTT and 4mmol/L LIS. The aneuploidy frequencies of chromosome 8 were significantly higher than the solvent control ( P <0.001) in both 240mg/kg group and 480mg/kg group. The aneuploidy frequecies were not significantly different between the sperm in 120mg/kg group and the solvent control ( P >0.05). It is concluded that THH could induce non disjunction of chromosome 8 in mouse sperm,and THH is a potential germinal aneugen in mammalian during the meiosis.更多还原