【摘要】 [目的 ]探讨黄芪注射液对低氧性肺动脉高压大鼠肺组织中超氧化物歧化酶与丙二醛的影响 .[方法 ]将 2 1只健康雄性Wistar大鼠随机分为 3组 ,每组 7只 .对照组 :室内空气正常饲养 ;低氧组 :在常压低氧舱内 (氧浓度 10 0 %± 0 5 %)缺氧 ,每日 8h ,连续 30d ;治疗组 :缺氧条件同低氧组 ,自缺氧第 1日起每天给大鼠腹腔内注射 12g·kg-1黄芪注射液 .每天往对照组与低氧组大鼠腹腔内注射的生理盐水量和治疗组相同 .于低氧 30d分别用右心导管法测定肺动脉平均压、颈动脉平均压 ,用生物化学方法测定肺组织中超氧化物歧化酶活性、丙二醛的含量 .[结果 ]低氧组肺动脉平均压、丙二醛含量较对照组明显升高 ,差异有显著性 ,治疗组与低氧组比较差异也有显著性 .肺组织中超氧化物歧化酶活性较对照组降低 ,差异有显著性 ,治疗组较低氧组升高 ,差异有显著性 .3组颈动脉平均压相比较差异无显著性 .[结论 ]慢性低氧导致肺动脉平均压上升、肺组织中氧自由基含量升高、超氧化物歧化酶活性下降 ,黄芪注射液可调节肺组织中超氧化物歧化酶活性与氧自由基含量 ,对肺动脉高压有较好的治疗作用 .更多还原

【Abstract】 OBJECTIVE?To study the influence of Radix Astragali on superoxide dismutase (SD) and malonyldialdehide (MDA) in pulmonary tissues of hypoxemic pulmonary hypertension in rats.?METHODS?21 healthy male Wistar rats were randomly divided into three group, 7 in each group. The control group was breed in common room condition, hypoxemic group in ordinary pressure hypoxia box (concentration of O 2 10 0%±0 5%) at 8 hours a day for 30 days and therapy group in the same condition as hypoxemic group and was received an intraperitoneal injection of Radix Astragali 12?g·kg -1 each day, but control and hypoxemic group were received same dose intraperitoneal injection of normal saline. The mean pulmonary arterial pressure, mean carotid pressure (mCAP) were measured by right cardiac catheterization, concentration of SD and MDA in pulmonary tissue was measured by radioimmunoassay.?RESULTS?mRAP and MDA in hypoxemic group were significantly higher than that in control group and there was a significant difference in therapy group as comparing with hypoxemic group. SD of pulmonary issue in hypoxemic group was lower than that in control and in therapy group. There was no difference on mCAP in three groups.? CONCLUSION ?The chronic hypoxia induces the elevation of mean pulmonary arterial pressure and oxygen free radical and descent of SD in pulmonary tissue. Injection of Radix Astragli could regulate the concentration of SD and oxygen free radical in pulmonary tissue and has good therapeutic effects for pulmonary hypertension.更多还原