【摘要】 目的　研究小檗碱 (Ber)与华法令 (War)、硫喷妥钠(Thi)、甲苯磺丁脲 (D860 )竞争血浆蛋白结合部位所致的药物相互作用。方法　采用体外透析平衡方法 ,观察Ber对War在透析外液中的浓度的影响 ;以药效学方法 ,观察Ber与War合用对小鼠凝血时间的影响 ;Ber与Thi合用对小鼠睡眠时间的影响 ;Ber与甲苯磺丁脲合用对小鼠血糖的影响。结果　Ber可明显增加透析外液中的War的浓度 (P <0 0 1) ;Ber与War合用 ,小鼠凝血时间明显延长 (P <0 0 1) ;Ber与Thi合用能明显延长小鼠睡眠时间 (P <0 0 1) ;Ber与D860 合用能明显降低小鼠血糖水平 (P <0 0 1)。结论　Ber能与War、Thi、D860 竞争血浆蛋白结合部位 ,使其游离药物浓度增高 ,药效 (或毒性 )增强更多还原

【Abstract】 AIM To study the interaction induced by berberine(Ber) displacing protein binding of warfarin(War),thiopental(Thi) and tolbutamide(D 860 ). METHODS Using the balance dialysis method, observed effect of Ber on War concentration in dialysate of dialysis tubing outside; using the slide method, observed effect of Ber on coagulation time of the mice administrated War; using right reflex method, observed effect of Ber on sleep time of the mice administrated Thi; determining level of blood glucose of the mice, observed effect of Ber on action of D 860 . RESULTS War concentration of the solution out dialysis tubing of War+Ber group were higher( P< 0 01), compared with War+PBS group. Ber can prolong coagulation time of the mice po War( P< 0 01). Ber can prolong sleep time of the mice ip Thi( P< 0 01). Ber can decrease the level of blood glucose of the mice po D 860 (compared with D 860 +NS group, P< 0 01). CONCLUSION Ber can displace War, Thi and D 860 from their protein binding site, increase the blood level of free drug of them, and enhance their actions or toxicity.更多还原