【摘要】 目的　探讨硫酸镍 (NiSO4)对大鼠海马神经细胞毒作用及其机制。方法　对海马神经细胞进行原代培养 ,观察不同浓度的NiSO40 0 0 1、0 0 1、0 1、1 0、10 0、10 0 0 μmol/L,对细胞增殖和分化的影响 ,并利用图像分析细胞形态的变化。结果　NiSO4明显抑制海马神经细胞分化和发育 ,集落形成率明显减少 ,细胞体积小 ,拟合细胞分化和增殖抑制曲线 ,计算得海马神经细胞半数分化抑制浓度 (ICD50 )为 9 5 μmol/L ,半数存活抑制浓度 (ICV50 )为 3 5 μmol/L。并显示浓度效应关系。结论　NiSO4抑制细胞分化、增殖可能是与其抑制蛋白质合成 ,并导致脂质过氧化有关。更多还原

【Abstract】 Objective To explore the developing toxicity and its mechanism of nickel sulfate (NiSO 4) on rat embryo hippocampal cells. Methods The effects of nickel sulfate on rat embryo hippocampal cell were studied at doses of 0.001, 0.01, 0.1, 1.0, 10.0, 100.0 μmol/L. The 16th day of rat embryo hippocampal cells were subjected to primary culture for 6 days. Results NiSO 4 inhibited differentiation of the cell with affecting cells’ proliferation. The concentration of 50% inhibitions of cell differentiation(ICD 50) was 9.5 μmol/L. The concentration of 50% inhibitions of cell proliferation(ICV 50) was 35 μmol/L. Conclusion The inhibition of nickel sulfate on embryo hippocampal cells might be associated with impact of protein synthetis, and lipid peroxidation.更多还原