【摘要】 对新型自动出凝血检测仪STA R(法国 ,Stago公司产品 )在儿科血液实验室应用进行评价。采用常规分析方法和儿科领域应用的评价方法。常规分析方法包括 :①准确性 ,含重复性 (n =2 1 ,2个质控血浆 )和可重现性(n =2 0 ,2个质控血浆 ) ,用于凝血酶原时间 (PT) ,活化部分凝血酶原激酶时间 (APTT) ,因子Ⅱ (FⅡ ) ,因子Ⅶ +Ⅹ(FⅦ +Ⅹ ) ,因子Ⅴ (FⅤ ) ,纤维蛋白原 (fg)和抗凝血酶 (AT)。②相关性 :选择 50份儿科病人新鲜血浆标本分别用STA R和STA检测和比较APTT ,FⅡ ,FⅦ +Ⅹ ,FⅤ和fg。儿科领域应用评价包括 :①低凝血因子浓度范围内评价STA R检测结果的准确性并比较STA R和STA的结果 ;②测定标本的最小容积 ;③特殊标本的应用评价。研究结果表明 ,STA R有很好的准确性。 50份血浆样本的STA R和STA检测结果密切相关 ,相关系数分别为 :APTT 0 .990 ;fg 0 .995 ;因子Ⅱ 0 .996 ;因子Ⅶ +Ⅹ 0 .997和因子Ⅴ 0 .996。在低凝血因子浓度范围得到了同样的结果。可接受的最小容积为 50 μl。很微量的标本、选择实验及急诊标本容易在本仪器操作。结论 :STA R显示了好的技术性能 ,特别适用于儿科出凝血指标的检查更多还原

【Abstract】 To evaluate the capacities of adaptation of the STA R analyzer (Diagnostica Stago, Asnieres, France) to the study of pediatric hemostasis, classical studies were undertaken and they have included: (1) within run assays (n=21, 2 control levels) and day to day precision studies (n=20, 2 control levels) for the following tests: prothrombin time (PT), activated partial thromboplastin time ( APTT ),factor Ⅱ(FⅡ), factor Ⅶ+Ⅹ(FⅦ+Ⅹ), factor Ⅴ(FⅤ), fibrinogen(fg) and antithrombin(AT); (2) comparison of the results of APTT, FⅡ, FⅦ+Ⅹ, FⅤ and fg assays (in 50 plasmas) obtained with STA R and STA (analyzer currently used, in our pediatric laboratory). Specific pediatric evaluation has included: (1) evaluation of the STA R results for low concentrations of the coagulation factors; (2) determination of the lowest dead volume; (3) introduction of peculiar samples among "routine" series. The results showed that within run assays and day to day precision studies have confirmed the good performances of the STA R. The comparison of the STA R and STA results from 50 plasmas shows a correlation coefficient of 0.990, 0.995, 0.996, 0.997 and 0.996 for APTT, fg, FⅡ, FⅦ+Ⅴ and FⅤ respectively. Such results are confirmed for low concentrations of coagulation factors. The acceptable dead volume could be lowered down to 50 microliters. Very small samples, selected assays and emergencies could be easily introduced among routine series. It is concluded that the STA R showed very good technical performances. The STA R is adapted to the routine tests as well as to the very specific samples of neonates and children.更多还原