【摘要】 目的研究苄基四氢巴马汀 (BTHP)和甲氧胺对豚鼠心室肌细胞上延迟整流钾电流作用。方法采用全细胞膜片钳技术测定延迟整流钾电流 (Ik)。结果甲氧胺 5 0 μmol·L- 1 在 37℃时可以分别使IK和IK ,tial从 (12 4± 1 8)pApF- 1 和 (4 2±0 5 )pApF- 1 上升至 (2 0 5± 0 7)pApF- 1 和 (7 2± 0 6 )pApF- 1 。若事先甲氧胺 5 0 μmol·L- 1 后 ,再加入BTHP 30 μmol·L- 1 可使BTHP阻滞IK和IK ,tial百分率分别从单独使用BTHP时的 37 5 %± 6 0 %和 35 9± 5 5 %上升到 6 2 9%± 4 3%和 6 1 1%±3 7%。结论苄基四氢巴马汀可以逆转甲氧胺对豚鼠心室肌细胞上IK 的增加作用。更多还原

【Abstract】 Objactive To investigate the effects of benzyltetrahydropalmatine and methoximine on delayed rectifier potassium current ( I k) in guinea pig ventricular myocytes Methods Whole cell patch clamp technique was used to record I k current Results Methoximine 50 μmol·L -1 augmented densities of I k and I k, tail from (12 4±1 8) pA pF -1 and (4 2±0 5 ) pA pF -1 to (20 5±0 7) pA pF -1 and (7 2±0 6) pA pF -1 respectively The effect of methoximine on the I k and I k,tail can be reversed by BTHP 30 μmol·L -1 , with the inhibition rates of 62 9%±4 3% and 61 1%±3 7%, respectively When the cells were exposed only to 30 μmol·L -1 BTHP, I k and I k,tail were blocked by 37 5%±6 0% and 35 9%±5 5% Conclusion The results show that BTHP can inhibit the enhancing effect of methoximine on I k in guinea pig ventricular myocytes $$$$更多还原