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用PAGE电泳法检测腹泻婴幼儿粪便中轮状病毒RNA
Detection of rotavirus RNA in fecal samples from diarrhea infantile by polyacrylamid gel electrophoresis
【摘要】 目的 探讨婴幼儿轮状病毒感染诊断方法。方法 用聚丙烯酰胺凝胶电泳法(PAGE)检测轮状病毒RNA。结果 41份腹泻婴幼儿粪便标本中有 16份为RNA阳性 ,阳性率为 39%。结论 用PAGE电泳方法检测轮状病毒RNA准确可靠 ,不易出现假阳性
【Abstract】 Objective To probe the dignostic method of rotavirus infection of infantile.Method Polyacrylamid gel electrophoresis (PAGE) was used to detect rotavirus RNA in fecal samples from infantile with diarrhoea.Result There was 16 positive of rotavirus RNA in 41 fecal samples from infantile with diarrhea,positive rate is 39%.Conclusion Detection of rotavirus RNA from fecal samples with PAGE is reliable and no false positive result.
【关键词】 轮状病毒;
聚丙烯酰胺电泳;
RNA;
婴幼儿;
腹泻;
【Key words】 rotavirus; polyacrylamid gel electrophoresis; RNA; infantile; diarrhea;
【Key words】 rotavirus; polyacrylamid gel electrophoresis; RNA; infantile; diarrhea;
【基金】 河南卫生厅资助课题
- 【文献出处】 新乡医学院学报 ,Journal of Xinxiang Medical College , 编辑部邮箱 ,2002年04期
- 【分类号】R446.13
- 【被引频次】12
- 【下载频次】193