【摘要】 目的　探索P物质对牛蛙背根神经节神经元γ -氨基丁酸激活电流抑制的机制或途径。方法　实验采用的方法是全细胞膜片钳技术 ,实验材料为急性分离的牛蛙背根神经节神经元。结果　P物质在牛蛙背根神经节神经元能引起一缓慢的内向电流 ;并且P物质能明显抑制牛蛙背根神经节神经元γ -氨基丁酸激活电流 ;蛋白激酶C的抑制剂H - 7(5 0ЧM)能阻断此抑制作用。结论　P物质可能通过蛋白激酶C抑制γ -氨基丁酸激活电流更多还原

【Abstract】 Objective To research the mechanism or pathway through which Substance P(SP) inhibits r-aminobutyric acid(GABA) activated currents in bullfrog dorsal root ganglion(DRG) neurons Method The experiment was conducted on freshly isolated bullfrog dorsal root ganglion neurons using a whole-cell patch-clamp technique Results SP could caused a slow inward current when SP was applied to DRG neurons;SP could inhibits GABA-activated currents;The inhibition could be reduced largely when protein kinase C(PKC) inhibiter,1-(5-isoquinolinesulphorry)-2-methylpiperazine(H-7), was dialyzed in cell body Conclusion The SP ton inhibit GABA-activated currents through protein kinase C.更多还原