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GNA和α-PAP双抗表达载体的构建及对烟草的遗传转化
Construction of binary expressing vector containing GNA gene andα-PAP gene and transformation of the vector into Nicotiana tabacum
【摘要】 将带有启动子GaMV35S、终止子Nos ter的雪花莲凝集素基因GNA插入到已构建好的植物表达载体pBI121/α PAP上,构建成双抗表达载体pBI121/GNA+α PAP,并采用农杆菌介导法将其转化进入烟草红花大金元,获得了卡那霉素筛选的抗性植株,通过PCR扩增验证,烟草基因组中含有这两个抗性基因。
【Abstract】 A plant expression vector with resistance to both virus and aphid was constructed by inserting Galanthus nivalis agglutinin gene(GNA)containing CaMV35S promoter and Nos terminal into pHI121／αPAP,a Pokeweed Antiviral Protein gene.GNA gene and αPAP gene were transferred into tobacco mediated by Agrobacterium tumefaciens and under Kanamycin selection pressure.The results of PCR from the kanamycin resistant transformants indicated that GAN gene and αPAP gene have been integrated into the tobacco genome.
【关键词】 雪花莲凝集素(GNA)；
商陆抗病毒蛋白(α-PAP)；
表达载体构建；
遗传转化；
【Key words】 Galanthus nivalis agglutinin(GNA)； Pokeweed Antiviral Protein (α-PAP)； expression vector； transformation；
【Key words】 Galanthus nivalis agglutinin(GNA)； Pokeweed Antiviral Protein (α-PAP)； expression vector； transformation；
【基金】 云南省应用基础研究(97C001G)
- 【文献出处】 西南农业学报 ,Southwest China Journal of Agricultural Sciences , 编辑部邮箱 ,2002年04期
- 【分类号】S188
- 【被引频次】33
- 【下载频次】110