【摘要】 目的 :建立急性病毒性心肌炎小鼠模型并检测其免疫功能指标。方法 :腹腔注射CVB3 建立急性病毒性心肌炎小鼠模型 ,用MTT比色法检测NK、TNF、IFN的活性 ;进行小鼠心肌组织病毒分离、病理检查 ,检测小鼠体重、心脏及脾脏重量变化。结果 :CVB3 感染鼠的体重及心脏、脾脏的重量均明显减轻 ;血清中TNF活性及脾细胞诱生TNF量显著增高 ;脾脏NK活性增高 ,但脾细胞诱生IL - 2量降低 ;IFN无明显变化。结论 :急性病毒性心肌炎模型小鼠的NK活性、细胞因子等指标均发生明显变化更多还原

【Abstract】 Objective: To geneate mouse model with myocarditis induced by Coxsackievirus B 3 and to detect the immune indices of the model mouse. Methods: Acute viral myocarditis was induced by injecting peritoneally CVB 3 in BALB/C mice. The activities of splenic NK, IFN-α TNF and serum IFN-I and TNF were measured by MTT assay and the content of splenic IL-2 was determined by ELISA method respectively. The weights of mouse and of its heart and spleen were recorded, CVB 3 in myocardium was identified by CPE protocol and mycardial lesion was pathologically checked. All data of experimental models and positive control models were collected and compared statistically. Results: The activities of Nk and TNF in murine model with Coxsackievirus B 3-induced myocarditis were significantly increased while the weight of heart and spleen and the content of splenic IL-2 sharply decreased, compared with those in healthy control group. No significant difference was found in the activity of IFN between the mouse model with coxsackievirus B 3-induced myocarditis and the healthy control group. Conclusions: The activity of NK and cytokine were involved in the development of myocarditis of experimental mice.更多还原