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重庆稻瘟病菌不同菌株DNA指纹图谱分析

ANALYSIS ON DNA FINGERPRINTS OF DIFFERENT ISOLATES OF MAGNAPORTHE GRISEA IN CHONGQING

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【作者】 杨水英肖崇刚韩海波杨静彭洪江何霞红

【Author】 YANG Shui-ying 1, XIAO Chong-gang 1, HAN Hai-bo 2, YANG Jing 3,PENG Hong-jiang 2, HE Xia-hong 3 (1. Department of Plant Protection, Southwest Agricultural University, Chongqing 400716,China; 2. Fulin Institute of Agricultural Science, Fulin Chongqi

【机构】 西南农业大学植保系涪陵农业科学研究所云南农业大学植病实验室云南农业大学植病实验室 重庆400716重庆400716重庆涪陵408000云南昆明650201云南昆明650201

【摘要】 采用rep -PCR指纹技术对 70个不同来源的稻瘟病菌菌株的DNA指纹图谱进行了测定。菌株间显示了DNA图谱的多态性 ,供试菌株分别扩增出 2~ 15条DNA带。经聚类分析 ,在 0 .80遗传相似水平下 ,供试菌株分为 9个遗传谱系 ,其中第 3,6 ,9为优势谱系。菌株的遗传谱系与菌株的原寄主和原采集地之间均表现出较明显的相关性

【Abstract】 DNA fingerprints of 70 Magnaporthe grisea isolates collected from different sources in Chongqing were tested using rep-PCR technique. Polymorphism of DNA amplified fingerprints appeared among the isolates. The number of DNA amplified bands varied from 2 to 15 in them. The isolated tested were clustered into 9 genetic lineages at 0.80 genetic similarity, of which No.3, No.6 and No.9 were the dominant lineages. Obvious relation was shown to exist between the genetic lineages of the isolates, on the one hand, and their original hosts and collecting places, on the other.

【基金】 国家 8 63计划项目 (国科生字 1 999[1 1 3]号 )
  • 【文献出处】 西南农业大学学报 ,Journal of Southwest Agricultural University , 编辑部邮箱 ,2002年03期
  • 【分类号】S432.4
  • 【被引频次】13
  • 【下载频次】80
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