【摘要】 对猪卵母细胞不同发育阶段的激素需要进行了初步探讨。结果表明 :猪卵母细胞体外培养 48h ,前 2 4h在培养液中加入激素 ,后 2 4h不加激素 ,卵母细胞的A级成熟率 (51 73% )和总成熟率 (83 2 5 % )最高 ,极显著高于前 2 4h不加激素 ,后 2 4h添加激素培养的成熟率 (P <0 0 1 ) ;也显著高于不含激素的培养液连续培养 48h的成熟率 (P <0 0 5) ;但与添加激素连续培养 48h组成熟率差异不显著 (P >0 0 5)。更多还原

【Abstract】 The hormone requirement in different developmental stages of porcine oocytes was studied. The results showed that when oocyte maturation for 48h in vitro, removal of hormone supplements from mature media at 24h after culture enhance oocyte maturation and cumulus expansion. The maturation rate (83 25%) was higher than that of oocytes that were cultured for 24h in the media without hormone subsequent supplement hormone for another 24h (63 94%) (P<0 01), and also higher than that of oocytes culture for 48h in media without hormones (60 01%) (P<0 05).更多还原