【摘要】 [目的]探讨用HeLa细胞HSP 70多肽诱导免疫活性细胞 (immunecompetentcells ,ICC)的特异性抗肿瘤机制。[方法]用热休克处理的HeLa细胞提取的HSP 70多肽剌激正常人外周血单个核细胞 ,在剌激扩增前后测定T淋巴细胞表型 ,并测定扩增的免疫活性细胞对HeLa细胞的杀瘤活性。[结果]在扩增的细胞中CD3+、CD8+淋巴细胞百分率明显增高 (P<0.01)。该免疫活性细胞对Hela细胞和来源于病人的宫颈癌细胞都具有较高的杀伤活性 ,而对经HSP 70单抗封闭后的HeLa细胞的杀伤率则明显下降。[结论]HeLa细胞HSP 70多肽能有效刺激PBMCs诱导活化免疫活性细胞 ,该免疫活性细胞具有特异性抗肿瘤作用更多还原

【Abstract】 To study the mechanism of anti tumor effect of the immune competent cells (ICC) induced by HSP 70 peptides of HeLa (cervical carcinoma) cells in vitro.The normal PBMCs were stimulated with HSP 70 peptides of HeLa cells in vitro.T cell phenotypes were analysed before and after PBMCs stimulation.The ICCs from PBMCs were tested for cytotoxic activity against HeLa cell.The percentages of CD3+ ?CD8+ cells were elevated significantly (P<0.01) in the expanded cells. The ICC induced by HSP 70 peptides had shown significant cytotoxic activity against HeLa cells and cercvical carcinoma cells from patients.The cytotoxic activity of the ICC against HeLa cells could be blocked by anti HSP 70 antibody.[Conclusion]The ICC can be induced selectively from PBMCs with HSP 70 peptides of HeLa cells, and has specific cytotoxic activity against HeLa cell.更多还原