【摘要】 以红莲型 (HL )细胞质雄性不育系和保持系为材料 ,构建了水稻线粒体基因组的 BAC文库。每个文库保存约2 30 0个菌落 ,外源插入片段介于 9～ 2 5 kb之间。以线粒体基因为探针对文库进行菌落原位杂交验证 ,均筛选到了阳性克隆。构建的两个文库为进一步研究水稻线粒体基因组的结构特点 ,为克隆与红莲型水稻细胞质雄性不育相关的线粒体基因奠定了基础更多还原

【Abstract】 Higher plant mitochondria plays a great role in the process of microspore development, and is closely associated with cytoplasmic male sterility (CMS). The library construction of mitochondrial genome is a necessary step to clone gene and research the structure of the genome. So here, the sterile line and maintainer lines of HL-type rice were used as materials, and BAC libraries of their genome were constructed. Each library consisted of about 2300 clones with insert size ranging from 9 to 25 kb. With mitochondrial genes as probes, positive clones were found. It was calculated that each library contains approximate 40 times of the rice mitochondrial genome.更多还原