【摘要】 根据甜菜抗线虫病基因序列 ,人工合成了三条上游引物和三条下游引物 ,从中筛选出一对引物 ,对 11份经抗线虫病常规鉴定的甘薯品种 (包括 6份高抗 ,2份抗病 ,3份感病 )进行了 PCR检测。结果表明 :6份高抗品种和 1份抗病品种出现了一条 6 30 bp左右大小的特异片段 ,而 3份感病品种未出现任何条带。这与常规鉴定的结果基本吻合 ,证明分子检测的可行性更多还原

【Abstract】 According to the sequence of nematode resistant gene in sugar beet, three forward primers and three reverse primers were produced by synthetic. A pair of primers were selected among them. 11 sweet potato cultivars (including 6 nematode high resistant, 2 resistant and 3 sensitive cultivars by conventional identification)were detected by PCR. The results showed that a special fragment of about 630 bp appeared in 6 high resistant and 1 resistant cultivars, and nothing in 3 infected cultivars. The result was in correspondence with that of conventional identification, and suggested the feasibility of PCR detection.更多还原