【摘要】 采用 RT- PCR和 n PCR扩增出 3株河南省近期 (1999～ 2 0 0 0年 )猪瘟流行野毒的 E2 基因 ,分别克隆于 PMD- 18T载体 ,对其进行了核苷酸序列测定及氨基酸序列推导 ,同时进行了同源性比较及 E2 糖蛋白结构的分析。结果表明 ,所测 3株 HCV E2 基因的长度均为 116 9bp。编码的氨基酸序列均包括完整信号肽序列和部分跨膜序列 ,共由 384个氨基酸组成。3株流行毒的 E2 基因核苷酸序列同源性在 99%以上 ,相应的氨基酸序列同源性也在 99%以上 ;这 3株流行毒与 C-株兔脾组织毒 (疫苗种毒 ) E2 基因的核苷酸序列同源性在 83.14 %～ 83.2 3% ,相应的氨基酸序列同源性在 88.14 %～ 88.6 8%。经遗传发生关系分析 ,C-株兔组织毒 C-株细胞毒属于组群 1(group 1) ,河南省近期流行猪瘟毒属于组群 2 (group 2 ) ,近期猪瘟流行毒与 C-株疫苗毒的 gp5 5蛋白之间存在一定的差异更多还原

【Abstract】 The E 2 gene of three prevalent virulent strains of Hog Cholera Virus (HCV) from Henan,China were amplified by reverse transcription(RT) and the nested Polymerase Chain Reaction (nPCR).The amplifed E\-2 fragments of three HCV strains were all 1 169 bp in length by agarose gel electrophoresis.Three E 2 fragments were cloned respectively into PMD 18T vector.1 169 bp cDNA fragments of three prevalent virulent E 2 gene were sequenced and 384 residues amino acid sequences of E 2 were deduced.The nucleotide homology among three prevalents virulents were over 99%;and that of amino acid were also 99%.The nucleotide identity of C strain from spleen tissue of rabbit with that of three Chinese prevalent virulent strains were from 83.14% to 83.33%,and that of amino acid were from 88.14% to 88.68%.There revealed some differences in gp55 of C strain and the 3 prevalent virulent strains.更多还原