【摘要】 低温处理小白菜 ( Brassica Campestrisssp.Chinensis var.communis Tsen et Lee)种子和春秋 6个播期实验 ,研究了小白菜春化和植株原位真空渗入遗传转化适宜苗态的建成方法。结果表明 ,小白菜种子经5 0℃温烫 1 0 min,2 3℃浸种 4 h,催芽 1 2 h后 ,在 0～ 4℃低温下 ,上海青处理 1 7d,黄心乌处理 2 5 d,四月慢处理 2 9d,7～ 9℃黄心乌处理 2 5 d,播种于大田 ,在长日照下均可完成春化 ,形成适宜植株原位真空渗入遗传转化的苗态。上海青、黄心乌和四月慢 9月 7日露地播种均可完成春化 ,形成适宜植株原位真空渗入遗传转化的最佳苗态 ,上海青和黄心乌 2月 1 7日播种也可完成春化 ,形成适宜植株原位真空渗入遗传转化的苗态。更多还原

【Abstract】 This paper investigated the method of forming suitable plant shape for in-planta agrobacterium mediated transformation with vacuum infiltration in pakchoi and the vernalization characteristics of pakchoi by treating pakchoi seeds under low temperature and sowing six times in spring and autumn. The experiment showed that the suitable plant shape would be formed and the plant completed vernalization would be attained when the seeds were warmed 10 min at 50 ℃, soaked 4 h and sprouted 12 h at 23 ℃, treated at 0~4℃ Shanghaiqing for 17 d, Huangxinwu for 25 d, Siyueman for 33 d, at 7~9 ℃, Huangxinwu for 25 d, then sowed in the open field under long daylight. When the three varieties are sowed on 7 september in open field, they would complete vernalization and form the optimum plant shape. When Shanghaiqing and Huangxinwu are sowed on 17 february in open field they would complete vernalization and form the suitable plant shape either.更多还原