【摘要】 介绍了一种用十二烷基磺酸钠 ( SDS)结合乙二胺四乙酸 ( EDTA)从圆褐固氮菌 G- 3细胞中分离聚 - β-羟基丁酸酯 ( PHB)的方法 ,并与 SDS单独作用菌体分离 PHB的方法作了比较 ,实验发现 ,前种方法具有更高的效率。主要研究了在不同试剂用量、作用时间、作用温度和 p H值等条件下 ,SDS和 EDTA提取 PHB的效果 ,并对分离条件进行了优化。结果表明 ,在 5 0℃ ,p H值为 7的条件下 ,用 1 0 g/L的 EDTA和 7g/L的 SDS共同作用菌体 (含 PHB66% ) 1 5 min,可使 PHB的纯度达到 98.75 %。更多还原

【Abstract】 The recovery of polyhydroxybutyrate (PHB) from Azotobacter chroococcum strain G 3 is studied.The two methods of EDTA cooperated SDS and SDS alone in recovery process were compared.It was discovered that the former was more efficient.The effect of some factors on extraction was discussed,and optimization was carried out.When 30 g/L cells having the PHB content of 66% was treated with 10 g/L EDTA and 7 g/L SDS at 50℃,pH=9 for 15 min,PHB was recovered with purity of 98.75%.更多还原