【摘要】 目的 :观察酒精引起的机体血液及组织自由基变化情况和自行组方中药的抗自由基作用。方法 :雄性SD大鼠持续白酒灌胃 4w后处死 ,测定血液及重要脏器超氧化物歧化酶 (SOD)及丙二醛 (MDA)含量 ,并对肝细胞的SOD1及乙醛脱氢酶 (NAD+ )基因表达做定性分析。结果 :与酒精组比较 ,中药组大鼠的血液及肌肉组织中MDA含量降低 ,SOD活性增高 ;与对照组比较 ,SOD1和NAD+ 的基因表达未见明显增强。结论 :血液及肌肉组织的自由基改变显著 ,组方中药具有明显清除自由基功能 ,但不能明显增强SOD1和NAD+ 基因的表达更多还原

【Abstract】 Objective:To observe free radical change in blood and tissue caused by alcohol consumption and antioxidant efficacy of Chinese decoction made by us.Methods:Male Sprague Dawley rats were decapitated after constant alcohol consumption for four weeks.Malondialdehyde(MDA) concentration and superoxide dismutase(SOD) activity were examined in the blood and important organ.Furthermore,SOD1 gene and NAD + gene expression were made quanlitive analysis in hepatic cell.Results:By contrast with alcohol group, we found that SOD activity in the blood and muscle tissue increased and MDA concentration decreased in the medicine treatment group.By contrast with control group, SOD1 and NAD + gene expression could not be activated.Conclusion:Free radicals in the blood and muscle tissue changed significantly, and Chinese decoction made by us can enhance the ability to eliminate free radicals and protect the body from alcohol toxicity, but SOD1 and NAD + gene expression could not be enhanced significantly.更多还原