【摘要】 应用高效液相色谱 电喷雾质谱联用法 ,直接对大豆胚芽的 70 %乙醇室温提取液进行检测 .根据各色谱峰的质谱特征 ,在室温提取液中鉴定出 9种异黄酮 ,7种A组皂苷 ,4种B组皂苷和2种E组皂苷 .结果证明对于复杂天然产物的定性定量分析 ,高效液相色谱 电喷雾质谱 (HPLC/ESI MS)联用分析法是一种方便快捷有效的方法 .更多还原

【Abstract】 Soybean isoflavones and saponins were extracted from soybean germs by 70% alcohol at room temperature, and determined using HPLC/ESI MS. According to their MS characters, nine kinds of isoflavones, seven types of group A saponins, four types of group B saponins and two types of group E saponins were identified. The results showed that HPLC/ESI MS was a convenient, rapid and effective method to analyze complicated nature products qualitatively and quantitatively.更多还原