【摘要】 为探讨一种快速简便的衰老动物模型 ,采用γ射线辐射SD大鼠后 ,测定其超氧化物歧化酶 (SOD)与丙二醛 (MDA)的变化及给小鼠颈背部皮下连续注射D 半乳糖后 ,测定其SOD与MDA的变化。结果表明(1)γ 射线辐照法 :SOD活力 (nU ml)实验组为 15 3± 46 ,对照组为 2 0 2± 6 0 ;MDA的含量 (nmol ml)实验组为 2 70± 75 ,对照组为 2 5 3 2± 2 0 8。辐照后两组的SOD活力和MDA含量有显著性差异 ,且SOD活力显著下降 ,MDA含量显著升高。 (2 )D 半乳糖法 :其结果与辐照法相同。提示在短期内用γ射线辐照的SD大鼠 ,能做为衰老动物模型更多还原

【Abstract】 In order to develop a quick and convenient model of acute induced-aging, γ-ray (3.0 Gy/time for 4 min 53s for 5 days ) is used to irradiate a group of rats, and galactose (40 mg·kg -1·d -1 for 40 days) is injected into a group of mice. At the end of the experiment, the activities of superoxide dismutase (SOD) and content of malondiadehycle (MDA) in the serum of experimental animals are assayed , showing that the γ-ray irradiation leads to the reduction of SOD activity and an increase in MDA content in the serum of the rats, while galactose injection leas to the reduction of SOD activity and an increase in MDA content in the serum of the mice. The results from this investigation indicate that an acute aging model can be induced by γ-ray irradiating on rats.更多还原