【摘要】 为了探讨铁对肥胖大鼠白色脂肪组织中UCP2及骨骼肌中UCP3基因表达的影响 ,应用RT PCR方法测定UCP2、UCP3mRNA表达水平。结果表明 :(1)适量补铁可改善机体甲状腺激素水平 ;(2 ) 5、10、2 0倍铁的高能饲料组肥胖大鼠的脂体比正常铁高能饲料组显著下降 (P <0 0 5 ) ;(3)缺铁使肥胖大鼠UCP2、UC P3mRNA表达水平明显降低 ;(4)补铁可使肥胖大鼠骨骼肌中UCP3mRNA表达水平增强 ,以 5倍 [(2 5 5 1mg (kg·d·BW) ]剂量铁组的UCP3mRNA表达水平最强 ,约是基础饲料组大鼠的 2倍 ;而对高能饲料诱导的白色脂肪组织中的UCP2基因表达增强无影响。结论认为适量补铁能改善机体甲状腺激素水平 ,促进肥胖大鼠骨骼肌中UCP3mRNA表达 ,但不能增加高能诱导的白色脂肪组织中UCP2基因表达更多还原

【Abstract】 In order to study the effect of iron on the expression of UCP2 in white fat and UCP3 in muscle in obese rats, a reverse transcription polymerase chain reaction (RT PCR) technique is used to measure the expression of UCP2 and UCP3 mRNA. The results show: (1)Adequate iron improves the level of thyroid hormone in blood serum;(2)the body fat content of obese rat in 5,10,and 20 times of iron supplement groups is lower than that of obese rats in normal iron supplement group;(3)the UCP2 and UCP3 mRNA expression of iron deficient obese rats is low in iron deficient obese rats;(4)iron supplement enhances the level of UCP3 mRNA expression in muscle and the level of UCP3 mRNA is the hightest in the 5 times iron supplement group and about 2 times of that of basic diet group.However, there is no effect on the UCP2 mRNA expression induced by high energy diet. It suggested that adequate iron can improve the level of serum thyroid hormone ,induce the UCP3 mRNA expression in muscle and mobilize fat to produce heat,but had no effect on UCP2 mRNA in white fat of obese rat.更多还原