【摘要】 根据嗜盐菌 (Halobacteriumsalinarum)NRC 3 4 0 0 1中硫解酶的基因序列信息 ,采用PCR技术从菌株Halobacteriumsp.ZP 6中克隆了极端嗜盐硫解酶的基因 ,并对此酶的氨基组成进行了分析。同非嗜盐硫解酶相比 ,极端嗜盐硫解酶不但含有较多的负电荷氨基酸 ,较少的正电荷氨基酸和强疏水氨基酸 ,而且同类氨基酸中的小氨基酸含量明显增高。这表明极端嗜盐硫解酶的嗜盐特性不单来自形成的分子静电屏蔽网和疏水作用的调节 ,且与分子表面张力减小密切相关。更多还原

【Abstract】 and 3′ end sequence of acaBl gene as primers, the gene of halophilic thiolase from haloarchae, Halobacterium sp. ZP 6 was cloned and its amino acid composition was calculated.Compared with non halophilic thiolase, the halophilic thiolase contains more negative charge amino acid, less positive amino acid and less strong hydrophobic amino acid, and use preferably small side chain amino acid. Those suggest that electrostatic screen, hydrophobic effect and surface tension all contribute to halophilic properties of thiolase.更多还原