【摘要】 FruB是与粘细菌 (Myxococcusxanthus)发育特异性转录因子FruA具有亲和力的蛋白因子 ,协同FruA参与对靶基因的调控。根据FruB氨基端氨基酸序列 ,设计简并性寡核苷酸引物对染色体DNA进行PCR扩增 ,以扩增产物为探针自粘细菌小型基因文库筛选出同源的4 5kbSacI阳性片段。fruB基因阻断分析表明 ,fruB功能缺失延缓子实体发育并降低粘孢子产率 ,提示fruB与粘细菌发育分化有一定联系更多还原

【Abstract】 FruB is an associated protein of FruA, a transcription factor essential for the development of Myxococcus xanthus. Degenerate oligonucleotide primers were designed based on the N-terminal amino acid sequence of FruB. Using genomic DNA as template, an approximate 110bp fragment was generated and further served as a probe to screen a small genomic library of M.xanthus. A 4.5kb SacI fragment was isolated on the basis of its homology to the probe. Disruption of fruB delayed the morphogenesis of fruiting bodies and depressed spore yield, suggesting that to some extent, FruB might be involved in the development of M.xanthus.更多还原