【摘要】 为筛选高效苯酚降解菌株 ,从炼油厂排污废水中分离筛选到 1株苯酚降解菌 phen8。利用PCR方法和琼脂糖凝胶电泳技术检测到 phen8菌中苯酚羟化酶基因片段的特异性条带 ,从基因水平上证实了 phen8菌的苯酚降解功能的遗传基础。应用PCR技术克隆到 16SrDNA片段 ,其核苷酸序列分析结果表明 ,该菌株的 16SrDNA全序列与斯氏假单胞菌DSM 5 0 2 2 7和DSM 5 0 2 38的同源性为 98% (在GenBank中的登记号为AF 2 8476 4)。初步确立了该菌在微生物系统发育学上的地位 ,暂定为假单胞菌 (Pseudomonassp .) phen8。更多还原

【Abstract】 A phenol-degradable strain named phen8 was isolated and screened from wastewater of an oil refinery in China. The function of phenol-degrading capability was examined at molecular level. PCR and electrophoresis analysis detected a typical band of DNA fragment of phenol hydroxylase. The results of plasmid analysis showed that genes encoding the enzymes degrading phenol are located in chromosome of the strain phen8. The complete sequence of the phen8 strain’s 16S ribosomal DNA was found to be homogeneous at the rate of 98% to the members of Pseudomonas stutzer DSM50227 and DSM50238 in GenBank of the Internet (registration number AF284764 in GenBank). Therefore, the position of the strain in microbiological phylogeny was established, for temporary it was classified as Pseudomonas sp phen8.;更多还原