【摘要】 通过鸟枪法构建了粘质沙雷氏菌SerratiamarcescensCW W 90 3菌株的基因组文库。使用LB 卵黄平板 ,从中筛选出 1条含磷酯酶基因的 3 0 10bp的EcoRⅠ片段。通过测序及亚克隆分析 ,发现 1个编码磷酯酶的基因phlA ,长度为 96 3bp ,编码 1个由 32 0个氨基酸组成 ,分子量为 33ku的磷酯酶PHL。PHL的氨基酸序列与多种细菌产生的磷酯酶A1的氨基酸序列有很高的同源性。在 phlA下游发现 1个 75 6bp的ORF phlB ,编码 1条 2 5 1个氨基酸组成的蛋白质 ,分子量为 2 7ku ,将其命名为PHLS ,此基因的功能有待于进一步研究。更多还原

【Abstract】 A genome library of Serratia marcescens CW-W-90-3 strain was established by “shot gun” method and screened for phospholipase activity with LB-egg yolk plate. A 3 010bp EcoRⅠfragment containing phospholipase gene was isolated. Further sequence analysis and subcloning revealed a 963bp phlA gene coding a 320AA phospholipase PHL with deduced molecular weight of 33KD. The PHL sequence shows high homology to the phospholipases A-1 from other bacteria. Another 756bp ORF was found downstream phlA gene, coding for a 251 AA polypeptide with molecular weight of 27KD, the protein was designated as PHLS,the function of this gene will be waiting for funther research.;更多还原