【摘要】 迄今报道的绝大多数纤维素酶的最适pH都在酸性和中性范围 ,当添加到洗涤剂中 ,由于处于碱性环境而无活力 ,不能发挥作用。近年来 ,国内外对由碱性芽孢杆菌 (Bacillussp )产生的碱性纤维素酶 (CMCase,endo β 1 ,4 glucanase,EC 3 2 1 4)进行了广泛的研究。对该酶产生菌株的筛选和培养条件、酶学性质 ,以及该酶基因的克隆和表达等方面的研究进行综述 ;并对我国目前未能实现该酶工业化原因进行了初步分析 ,并提出解决途径。更多还原

【Abstract】 The great majority of cellulases, so far reported, have optimal pH in the acidic or neutral range, which have no activity in detergent because of the alkaline condition of detergent compositions. Recently, researchers have given extensive attention to the alkalophilic Bacillus species which were found to produce an alkaline carboxymethyl cellulase (CMCase, endo-β-1,4-glucanase, EC 3. 2. 1. 4). In this paper, we reviewed the progress of strain selection and the conditions of incubation, its enzymology properties, its gene clone and expression. We also analyzed the reasons that the enzyme hasn’t been realized the industrial production in our country and give some proposals of the resolving way.更多还原