【摘要】 用HPLC和TLC测得冰川棘豆中的主要毒性成分2,2,6,6-四甲基-4-哌啶(2,2,6,6-tetramethyl-4-piperidone,TMPD)的含量为0.082～0.085%,另外两种生物碱的含量分别为0.0097%和0.0051%。用高压液相色谱法测定TMPD含量的条件以ODS C18 (4.6 mm×4.5 cm)反相柱,MeOH+H2O=97+3流动相,215 nm检测为宜。在硅胶G板上TMPD与Ehrlich试剂加热显色物的最大吸收波长为488 nm,最低检出限量为7.8 μg;在试管中TMPD与2%柠檬酸(Ac2O)液沸水浴显色物的最大吸收波长为550 nm,其最低检出限量为650 ng。更多还原

【Abstract】 With methods of high pressure liquid chromatogram (HPLC) or thin layer chromatogram (TLC), the content of 2,2,6,6-tetramethyl-4-piperidone (TMPD) in Glacial crazyweed (Oxytropis glacialis) was 0.082～0.085% and the contents of other two unknown alkaloids were only 0.0097% and 0.0051% respectively. HPLC conditions were, columniation: ODS C18 (4.6 mm×4.5 cm), flowing phase: methyl : H2O = 97+3, wavelength of the inspector: 215 nm.. On gel plate, TMPD appeared color with Ehrlich`s reagent when heated with maximum absorb wavelength 488 nm. This method could determinate TMPD when its quantity was higher than 7.8 μg. And, in the test tube, TMPD appeared color with 2% citric acid in acetic anhydride reagent when heated in boiling water with maximum absorb wavelength 550 nm.. The method could determinate TMPD when its quantity was higher than 650 ng.更多还原