【摘要】 采用天然海水配制的台盼蓝染色液和曙红B染色液对低温冷冻的锯缘青蟹精子的活力进行评价研究 .结果表明 ,用这两种染色液染色死活精子呈现出明显不同的特征 ,进而以无钙离子人工海水 (Ca2 + FSW )为冷冻基础液 ,分别用 1 0 %(V/V)甘油、5%(V/V)二甲亚砜 (DMSO)作冷冻剂 ,进行了 4℃液态低温保存的精子实验 ,对照组不加冷冻剂 ,以Ca2 + FSW等量代替 ,同时采用染色法对保存精子活力进行评价 ,结果显示台盼蓝不适宜于评价甘油组精子的活力 ,但却能评价对照组和二甲亚砜组 .在 2 4h内 ,二甲亚砜组精子的存活率达 58.7%,而对照组仅为 3 0 .2 %.可见 ,染色法可以作为低温冷冻保存的锯缘青蟹精子活力评价的一种有效方法 .更多还原

【Abstract】 The viability assay of spermatozoa of mud crab (Scylla serrata) is carried out by means of the traditional biostain methods of tryphan blue and erosin B.Spermatozoa are frozen under 4℃.Three samples are extended by the Ca 2+-FSW,10%(V/V)glycerol,5%(V/V)DMSO,Ca 2+-FSW is the control. With the tryphan blue,we cannot assay the sample extended by the glycerol,but we can easily distinguish the samples extended by the Ca 2+-FSW and the DMSO. After 24 h, the cryopreserved spermatozoa sample extended by the 5% (V/V)DMSO has 58.70% viability,while the control only has about 30.2% viability. These affirm that the tryphan blue biostain can be used to assay the viability of spermatozoa in a certain extent.This result is a valuable reference for us to do some genetic work and freezing research in spermatozoa of edible mud crab.更多还原