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杜仲药用有效成分提取方法研究
STUDY ON THE OPTIMUM EXTRACTION TECHNOLOGY OF EUCOMMIA ULMOIDES OLIV.
【摘要】 为优选杜仲的提取工艺条件 ,本实验以杜仲提取液中氯原酸的含量和浸膏得率为指标 ,采用四因素三水平正交设计法 ,筛选影响杜仲提取工艺的因素 ,探索最优的提取工艺条件。实验证明合理的提取方法为 :12倍 6 0 %的乙醇回流 3次 ,每次 2小时 ;所得杜仲提取物中氯原酸含量为 8 72mg/g生药 ,浸膏得率为 2 0 6 8%。
【Abstract】 Eucommia ulmoides Oliv.is a traditional Chinese herbal medicine.In order to optimize the extraction technology of Eucommia ulmoides Oliv.,We took total content of chlorogenic acid and extraction as inspecting index,used orthogonal experiment design(4 factors and 3 levels)to determine the extraction technology.The result indicates the optimum extraction technology is adding 12 times amount of 60% alcohol,refluxing for 3 times,2 h each time,this extraction technology show higher yield of Eucommia ulmoides Oliv.and good stability.
【关键词】 杜仲;
氯原酸;
正交实验;
提取工艺;
【Key words】 Eucommia ulmoides Oliv.; chlorogenic acid; orthogonal design; extraction technology;
【Key words】 Eucommia ulmoides Oliv.; chlorogenic acid; orthogonal design; extraction technology;
- 【文献出处】 天然产物研究与开发 ,Natural Product Research and Development , 编辑部邮箱 ,2002年06期
- 【分类号】R284
- 【被引频次】32
- 【下载频次】856