【摘要】 目的　探讨医用生物胶中耳腔局部应用对豚鼠内耳组织的影响 ,为临床安全使用提供实验依据。方法　 40只豚鼠分为医用生物胶组和生理盐水对照组 ,在耳蜗圆窗龛处滴注约 5 0 μl医用生物胶 (实验组 )及 5 0 μl生理盐水 (对照组 ) ,饲养 6周后 ,用ABR测试耳蜗生理功能 ,耳蜗铺片计算毛细胞的损伤缺失率 ,扫描电镜观察耳蜗毛细胞变化。结果　实验组和生理盐水对照组ABR测试两组间比较无显著性差异 ,两组耳蜗铺片显示毛细胞及支持细胞结构正常 ,缺失率小 ,两组间比较无显著性差异 ,电镜观察两组毛细胞结构正常。结论　医用生物胶中耳腔局部应用对内耳组织无损伤 ,说明对内耳无毒副作用更多还原

【Abstract】 Objective To investigate the effect of medical biological glue on inner ear when used locally in the middle ear.Methods Forty guinea pigs were randomly divided into experimental group and control group .50 ul medical biological glue was dropped in round window nitch.Auditory brainstem response (ABR) was carried out to evaluate cochlear physical function. Cochlea surface preparation was employed to count the loss rate of hair cells.Scanning electron microscope was used to observe hair cells pattern.Results ABR records as well as cochlea surface preparation showed no statistical difference between experimental and control group, scanning electron microscope didn’t demonstrate abnormal hair cells pattern.Conclusion This experiment indicates that topical application of medical biological glue in the middle ear has no harmful effect on cochlea.更多还原