【摘要】 采用一种新型氯甲酸酯类荧光衍生试剂咔唑_9乙基氯甲酸酯（CEOC）对氨基酸进行柱前衍生,并通过荧光检测的方法进行高效液相色谱 （HPLC）分析 ;在HypersilBDSC18 柱上对19种氨基酸衍生物进行了分离 ,衍生物的激发和发射波长分别为λex=293nm ,λem=365nm ,当衍生化缓冲液pH为9.0,衍生试剂总量 （摩尔数）为氨基酸总量的4倍时 ,衍生化产率最大 ,平均检出限可达22.8fmol。更多还原

【Abstract】 A new chloroformate fluorescence reagent, 2-(9-carbazol)-ethyl -chloroformate(CEOC), is used for the analysis of amino acids. In a one-step procedure, CEOC reacts with amino acids to form stable derivatives. Maximal yields of derivatization are observed with the amount of the derivant reagent being equal to 4 times of amount of amino acids. The method in conjunction with a gradient elution offers a baseline separation on a reversed_phase Hypersil BDS C18 column. The fluorescent derivatives are detected with a fluorescence monitor at λex of 293 nm and λem of 365 nm. The average detection limits reaches 22.8 fmol.更多还原