【摘要】 提出了测定加替沙星的荧光光度法 ,并对影响加替沙星荧光性质的各种因素进行了研究 ;加替沙星浓度在3.0×10-7～1.0×10-5 mol/L范围内与荧光强度呈良好的线性关系,检出限为8.8×10-8 mol/L ,对5.0×10 -7mol/L的加替沙星平行测定13次的相对标准偏差为1.4 % ;将该法用于血清、尿样分析 ,回收率在98%～103% ,结果令人满意。更多还原

【Abstract】 A fluorimetric method for the determination of gatifloxacin (GFLX) was proposed, and the main factors affecting on fluorescence of GFLX were studied. The linear relationship between the fluorescence intensity and concentration of GFLX was over the range of 3.0×10-7～1.0×10-5 mol/L. The detection limit was 8.8×10-8 mol/L. A relative standard deviation of 1.4% was obtained in a repeatability study(n=13) for a standard solution of GFLX at a level of 5×10-7 mol/L. The recommended method was successfully applied to the determination of GFLX in human serum and urine. The recoveries were in the range of 98%～103%, and the analytical results were satisfactory.更多还原