【摘要】 目的 :从细胞水平探讨精索静脉曲张大鼠睾丸线粒体损害的活性氧 (ROS)机制。方法 :将精索静脉曲张模型组 (VG)和假手术组 (SOG) Wistar大鼠于术后 3个月 ,取双侧睾丸 ,制作超薄切片和分离线粒体 ,各指标测定按试剂盒说明书进行。结果 :VG左、右侧睾丸线粒体丙二醛 (MDA)含量分别高于 SOG(P<0 .0 1 ) ,Na+ - K+ - ATPase、Ca2 + - ATPase、GSH- Px活力分别低于 SOG(P<0 .0 1 ,P<0 .0 5 ) ,SOD活力两组间无显著性差异 (P>0 .0 5 )。相关检验显示 VG左侧睾丸线粒体的MDA含量分别与其 Na+ - K+ - ATPase、Ca2 + - ATPase、GSH- Px活力呈负相关 (r=- 0 .71 8,r=- 0 .6 5 6 ,r=- 0 .6 3 6 ,P<0 .0 5 )。电镜发现 :精索静脉曲张大鼠双侧睾丸均有不同程度的病变 ,以线粒体改变为主要特征 ,且静脉结扎侧较重。结论 :静索静脉曲张时线粒体 ROS产生增加、清除发生障碍 ,线粒体结构、功能改变将导致睾丸功能紊乱 ,进而造成不育更多还原

【Abstract】 Objective: To investigate the mechanism by which ROS’ mitochondrial damage of testes in rats with varicocele on cell level. Materials & methods: Adult male Wistar rats were randomly divided into 2 groups i.e, varicocele group (VG) and sham operation group(SOG). After three months, bilateral testes were removed to make ultrathin section and their mitochondria were isolated. Index’s determing was according to the direction of kit. Results: Mitochondrial MDA content of bilateral testes in VG were higher than those in SOG (P< 0.01,P< 0.05), Na +-K +ATPase, Ca 2+-ATPase and GSH-Px activity were lower in VG than those in SOG (P< 0.01,P< 0.05). Result of correlation analysis showed that left testicular mitochondrial MDA content in VG was negatively correlated with Na +-K +-ATPase,Ca 2+-ATPase,GSH-Px activity respectively (r= -0.718, r=-0.656, r=-0.636, P< 0.05). Mitochondrial SOD activity between two groups had no significant differences. Different pathological change of bilateral testes were found in VG, characterized by mitochondrial change, and obvious in left ones by using transmission electron microscope. Conclusions: Increased ROS, disturbance of elimination and changes of mitochondrial construction and function can cause testes function disorder and thus lead to infertility.更多还原