【摘要】 目的　观察基质金属蛋白酶 MMP- 2和 MMP- 9在子宫颈癌组织中的表达 ,探讨其与子宫颈癌浸润及转移的关系。方法　采用免疫组化方法对 30例子宫颈鳞癌 ,2 9例子宫颈上皮内瘤样病变 (CIN)和 16例正常子宫颈组织进行标记及分析。结果　 MMP- 2、MMP- 9在子宫颈鳞癌、CIN、正常宫颈组织细胞浆中的阳性表达分别为2 3/ 30 (76 .7% )、17/ 30 (5 8.9% ) ;13/ 2 9(4 4.8% )、18/ 2 9(6 2 .1% ) ;4/ 16 (2 5 .0 % )、4/ 16 (2 5 .0 % )。 MMP- 2鳞癌组与CIN组及正常组相比差异有极显著性 (P<0 .0 1)。 MMP- 9正常组与鳞癌组及 CIN组相比差异有显著性 (P<0 .0 5 )。MMP- 2在各组间质的基质细胞中表达均为阳性。MMP- 9在正常宫颈组织、CIN及鳞癌的基质细胞中阳性表达分别为 0 / 16 (0 % ) ;4/ 2 9(13.8% ) ;7/ 30 (2 3.3% )。各组间相比差异无显著性。MMP- 2和 MMP- 9在鳞癌细胞浆中的阳性表达与肿瘤大小、临床分期和病理分化程度均无明显相关性。结论　 MMP- 2和 MMP- 9在子宫颈鳞癌组织中的表达与正常宫颈组织相比差异均有显著性。MMP- 2和 MMP- 9的表达水平的上调可能是子宫颈鳞癌侵袭的一个早期标志 ,但不能单独作为预测子宫颈癌结局的一个标志。更多还原

【Abstract】 Objective To observe the expression of matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9 (MMP-9) in human cervical carcinomas,to clarify the relationship between their expression and invasion,metastasis of human cervical carcinoma. Methods Tissue specimens were stained immunohistochemically for MMP-2 and MMP-9 in 30 cases of squamous cell cervical carcinoma,29 cases of cervical intraepithelial neoplasia (CIN) and 16 cases of normal cervices. Results MMP-2 and MMP-9 were positive in 23/30 (76.6%) and 17/30 (58.9%) of carcinomas,in 13/29 (44.8%) and 18/29 (62.1%) of CIN lesions respectively and both of them were positive in 4/16(25.0%) of normal cervices. There were significant differences for MMP-2 between carcinomas and normal cervices and CIN respectively ( P <0.01).There was significant difference for MMP-9 between normal cervices and carcinoma,between carcinomas and CIN respectively ( P <0.05).Peritumoral positive stromal cells for MMP-2 were observed in all cases.Peritumoral MMP-9 positive stromal cells were observed in 7/30(23.3%) of carcinomas,in 4/29(13.8%) of CIN,but was uninformly negative from normal cervices.No significant correlation could be established between the expression of MMP-2 and MMP-9 and the clinical stage and pathologic grade. Conclusion The expression of MMP-2 and MMP-9 can be possibly an early marker of tumor progression in squamous carcinoma of the cervix,but they do not appear to predict disease outcome.\;更多还原