【摘要】 目的 :探讨生长因子在慢性阻塞性肺疾病 (COPD)支气管重塑中的作用。方法 :采用SP免疫组织化学方法观察转化生长因子 β(TGF β)和表皮生长因子 (EGF)在 1 1例COPD患者纤维支气管镜活检标本组织中的表达 ;1 0例仅有咯血 ,无心肺疾病及非吸烟者为对照组。结果 :①TGF β和EGF在对照组支气管上皮细胞无表达 ;在COPD组支气管黏膜上皮细胞有明显的表达并与对照组相比差异有显著性。TGF β支气管黏膜上皮细胞基底膜是主要阳性表达区 ,化生黏膜上皮有较多阳性细胞。而EGF表达主要在纤毛上皮、支气管黏膜上皮细胞基底膜和化生黏膜上皮细胞。②在对照组黏膜下层有较少表达TGF β和EGF的阳性细胞。COPD组TGF β和EGF表达强 ,主要位于黏膜下层浸润的细胞、平滑肌细胞、黏膜腺体和内皮细胞。TGF β在细胞外基质和上皮下基底膜的胶原带有强表达。③在COPD组Vimentin 阳性细胞即成纤维细胞数明显增多 ,与对照组相比差异有显著性 (P <0 0 0 1 )。④黏膜上皮和黏膜下TGF β阳性细胞表达与基底膜厚度呈正相关(r=0 6 4 9P <0 0 1 ,r =0 6 4 9P <0 0 1 )。TGF β阳性细胞表达数和黏膜下层纤维细胞数呈正相关 (r =0 6 89P <0 0 1 ) ;EGF阳性细胞表达与基底膜厚度和黏膜下层纤维细胞数无相关性。结论 :TGF β可能参?更多还原

【Abstract】 Objective: To explore the roles of growth factors in remodeling of bronchia of chronic obstructive pulmonary disease (COPD). Methods: Expression of Transforming Growth Factor-β(TGF-β) and Epidermal Growth Faotor (EGF) was investigated in bronchial mucosal biopsies from 11 patients with COPD by immunohistochemical staining. 10 normal subjects who had only hemoptysis without any respiratory and heart dieases were selected as controls. Results: ① TGF-β and EGF were rarely expressed in epithelial cells from controls. In comparison with the controls, there were a greatly and highly significant increase (P<0.001) in COPD. TGF-β positive staining was mainly observed on basal bronchial epithelial cells, and the number of positive cells and the intensity of the staining was great in metaplastic epithelium. EGF staining was observed on ciliated and basal bronchial epithelial cells and in the metaplastic epithelium. ② The number of cells expressing TGF-β and EGF were low in controls and significantly great in COPD (P<0.001). Their immunostaining were localized on cells infiltrating submucosa, smooth muscle cells, mucous glands and endothelial cells. Moreover, positive immunostaining for TGF-β was localized in subepithelial collagen zone of the basal membrane and in the extracellular matrix. ③ Vimentin-cell standing for the number of fibroblasts was greatly and significantly increased in COPD compared to controls (P<0.001). ④ The increased expression of TGF-β was significantly correlated with the thickness of the basal membrane and the number of fibroblasts (P<0.01). The positiue expression of EGF was no correlation with the thickness of the basal membrane and the number of fibroblasts. Conclusion: TGF-β may be an important growth factor which involved in airways remodeling in COPD.更多还原